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Cutting-edge research in microbial ecology requires detailed understanding of microbiome diversity. Metagenomics approach and high-throughput sequencing technologies generated major breakthroughs in the field, but integration of all sequences stored in databases is urgently needed. The aim of the IMNGS project is to provide to the scientific community a unique and freely available platform for global assessment of 16S rRNA gene diversity in a broad range of microbial ecosystems. The IMNGS platform uniformly processes all 16S rRNA amplicon datasets from SRA to enable assessment of the distribution and diversity of specific bacteria of clinical or ecological relevance. Using a web front, this massively integrated sequence resource can be easily queried by users to address questions of relevance in microbial ecology. For additional information please refer to the original publication.